Biochemical impact of solar radiation exposure on human keratinocytes monitored by Raman spectroscopy; effects of cell culture environment

Understanding and amelioration of the effects solar radiation exposure are critical in preventing occurrence skin cancer. Towards this end, many studies have been conducted 2D cell culture models under simplified unrealistic conditions. 3D better capture complexity vivo physiology, although extracellular matrix not well studied. Monitoring instantaneous resultant cellular responses to exposure, influence environment, could provide an enhanced understanding fundamental processes photocarcinogenesis. This work presents analysis biochemical impacts simulated (SSR) occurring immortalised human epithelial keratinocytes (HaCaT), a model, compared culture. Cell viability was monitored using Alamar Blue colorimetric assay (AB), impact at level biomolecular constituents (nucleic acids proteins), were evaluated through combination Raman microspectroscopy multivariate statistical analysis. The results suggest that SSR induces alterations conformational structure DNA as immediate impact, whereas changes protein signature primarily seen subsequent response.